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Image Search Results
Journal: BioMed Research International
Article Title: Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells
doi: 10.1155/2018/3171348
Figure Lengend Snippet: Cell apoptosis observed using fluorescence microscope (200×). Cells were treated with different dose of govaniadine for 24 h and stained with (row (a)) acridine orange-ethidium bromide and (row (b)) Hoechst 33258. (A and A′) untreated cells (0.1% DMSO), (B and B′) cells treated with 2 μ M of govaniadine, (C and C′) cells treated with 4 μ M of govaniadine, (D and D′) cells treated with 8 μ M of govaniadine, and (E and E′) cells treated with 16 μ M of govaniadine. Scale bar: 100 μ m.
Article Snippet: After 24 h, apoptosis mediated by the govaniadine on MCF-7 cells was detected by the
Techniques: Fluorescence, Microscopy, Staining
Journal: BioMed Research International
Article Title: Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells
doi: 10.1155/2018/3171348
Figure Lengend Snippet: Induction of apoptosis in MCF-7 cells by govaniadine determined by Annexin V-PI flow cytometry technique. (a) Control (0.1% DMSO), (b), (c), and (d) treated with govaniadine 4, 8, and 16 μ M, respectively. The lower left quadrant represents intact viable cells (Annexin-FITC and PI negative). The upper left quadrant represents early apoptotic cells (Annexin-FITC positive and PI negative). The upper right region represents late apoptotic cells (Annexin-FITC negative and PI positive). The lower left quadrant represents necrotic cells (Annexin-FITC and PI positive). The data are presented as dot plots of Annexin V/FITC against PI of at least three independent tests. Data were analyzed by specific software, FCS Express 5.
Article Snippet: After 24 h, apoptosis mediated by the govaniadine on MCF-7 cells was detected by the
Techniques: Flow Cytometry, Control, Software